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链霉亲和素树脂,货号:786-390
链霉亲和素树脂
货号:786-390
英文名: Streptavidin Resin(Settled Resin)
相关类别: 亲和纯化介质
储存: 冷藏(2-8℃)
规格:5 ML RESIN
概述
Immobilized Streptavidin Resin is designed for the affinity chromatography purifications, assay development and immunopreciptations of proteins, antibodies and other molecules with a biotin tag. The resin consists of streptavidin coupled to 6% cross-linked agarose.
特点
- Streptavidin is a tetrameric protein and in many respects is similar to avidin except that it has no carbohydrate and has a slightly lower molecular weight of about 60kDa. The advantage of streptavidin is that the lack of carbohydrates significantly reduces the amount of non-specific binding.The solubility of streptavidin (isoelectric pH5) in aqueous buffer is much lower than avidin, but the binding to biotin is similar.
应用
Application for immunoprecipitation with biotinylated antibodies; Pull down assays with biotinylated proteins; Purification of biotinylated molecules including proteins, antibodies, DNA, carbohydrates.
组份
5 ml resin
技术规格
| Bead Structure | 6%Cross linked agarose for minimal leaching |
| Streptavidin Density | >1mg/ml/ml packed resin |
| Binding capacity | ≥15-30 μg biotin/ml resin |
| Stability Range | pH 2~11 |
| Preservative | 50% slurry in PBS |
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