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梭菌蛋白酶(胞内蛋白酶-精氨酸-羧基)CAS : 9028-00-6

    

梭菌蛋白酶(胞内蛋白酶-精氨酸-羧基)CAS : 9028-00-6

英文名: Clostripain (Endoproteinase-Arg-C)

货号:zk4323
别名: Endoproteinase-Arg-C
级别Activity ≥50 U/mg dw
储存冷藏(2-8℃)
规格:1mg 5mg 

概述

Clostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from?Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory.

技术规格

Source Clostridium histolyticum
Description Chromatographically purified. A dialyzed, lyophilized pre-activated powder.
Storage Store at 2-8°C
Activity ≥50 units per mg dry weight
Unit Definition One Unit hydrolyzes one micromole of N-benzoyl-L-arginine ethyl ester per minute at 25°C, pH 7.6, in the presence of dithiothreitol.

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