中性蛋白酶，分散酶，已纯化CAS : 78990-62-2

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中性蛋白酶，分散酶，已纯化CAS : 78990-62-2

货号：zk6118

英文名： Neutral Protease (Dispase), Purified
别名： Dispase; 分散酶
级别： Activity ≥4 U/mg dw
储存：冷藏（2-8℃）分子量： 36 kDa
规格：10mg 50mg 250mg

概述：

Neutral protease (Dispase?) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at our company. Its mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) cell culture, since it is gentle on cell membranes. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications, commonly used with collagense.

技术规格：

pH Optimum	Stable over a wide pH range: 4.0-9.0, optimum pH 5.9-7.0
Stability	Stable at 2-8°C for 12 months. Aliquot and store at -20°C after reconstitution with water or commonly used balanced salt solutions or media
Specificity	Non-specific cleavage of peptide bonds containing leucine and phenylalanine.
Activators	Divalent cations including Ca2+, Mg2+, Mn2+, and Fe2
Inhibitors	EDTA (1mM), EGTA, 1-10-phenanthroline and heavy metals
Application	Commonly used to separate skin epidermis from dermis leaving intact epithelial sheets and stem cell, hepatocyte and other cell isolation applications. However, due to the diversity of the variables involved, exact isolation conditions should be determined empirically for each cell/tissue application.
Source	Bacillus polymyxa
Description	Chromatographically purified. A lyophilized powder.
Storage	Store at 2-8°C.
Activity	≥4 units per mg dry weight
Unit Definition	One Unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using casein as the substrate.

中性红CAS : 553-24-2

乙酸镍四水CAS : 6018-89-9

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