Mouse IL-6 Quantikine ELISA Kit

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Mouse IL-6 Quantikine ELISA Kit

Mouse IL-6 Quantikine ELISA Kit Summary

Catalog Size / Price
M6000B 1 Kit (2 Plates) / Please Inquire
SM6000B 1 Pack (6 Plates) / Please Inquire

PM6000B 1 Pack (50 Plates) / Please Inquire

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL)

Sensitivity

1.8 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)

Specificity

Natural and recombinant mouse IL-6

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-6. Results obtained using natural mouse IL-6 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	28	22	27
Mean	30	87	232	29	85	238
Standard Deviation	2	3.4	8.1	1.8	7.5	18.1
CV%	6.7	3.9	3.5	6.1	8.9	7.6

Recovery

The recovery of mouse IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=5)	110	86-120
Heparin Plasma (n=6)	101	81-115
Serum (n=5)	99	84-113

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