Mouse IFN-gamma Quantikine ELISA Kit

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Mouse IFN-gamma Quantikine ELISA Kit

Mouse IFN-gamma Quantikine ELISA Kit Summary

Catalog # Availability Size / Price
MIF00 1 Kit (2 Plates) / Please Inquire
SMIF00 1 Pack (6 Plates) / Please Inquire

PMIF00 1 Pack (50 Plates) / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL)

Sensitivity

2 pg/mL

Assay Range

9.4 - 600 pg/mL (Cell Culture Supernates, Serum)

Specificity

Natural and recombinant mouse IFN-gamma

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IFN-gamma in cell culture supernates and mouse serum. It contains E. coli-expressed mouse IFN-gamma and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse IFN-gamma accurately. Results obtained using natural mouse IFN-gamma showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-gamma.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	45.1	143	528	42.8	139	518
Standard Deviation	2.1	4.4	11.8	3.6	13.6	46.5
CV%	4.7	3.1	2.2	8.4	9.8	9

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	44.8	156	518	43.3	144	509
Standard Deviation	2.2	5	14.2	3.6	14.7	48.6
CV%	4.9	3.2	2.7	8.3	10.2	9.5

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