Human IL-10 Quantikine ELISA Kit

Human IL-10 Quantikine ELISA Kit Summary

货号：D1000B

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)

Sensitivity

3.9 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human IL-10

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-10 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-10 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-10 and does not cross-react with viral IL-10. Results obtained using natural human IL-10 showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate this kit can be used to determine relative mass values for natural human IL-10.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	24.4	79.6	223	23.6	74.2	227
Standard Deviation	1.6	3.9	5.5	1.8	4.9	12.7
CV%	6.6	4.9	2.5	7.6	6.6	5.6

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	23.9	76.9	231	23.2	75.1	228
Standard Deviation	1.2	3.3	3.9	1.7	5.6	13.4
CV%	5	4.3	1.7	7.3	7.5	5.9

Recovery

The recovery of human IL-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	98	88-110
Citrate Plasma (n=5)	96	87-108
EDTA Plasma (n=5)	92	75-105
Heparin Plasma (n=5)	94	84-105
Serum (n=5)	100	92-110

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of IL-10 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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