Mouse/Rat Leptin Quantikine ELISA Kit

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Mouse/Rat Leptin Quantikine ELISA Kit

Mouse/Rat Leptin Quantikine ELISA Kit Summary

货号:MOB00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (20 uL), EDTA Plasma (20 uL)

Sensitivity

22 pg/mL

Assay Range

62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)

Specificity

Natural and recombinant mouse and rat Leptin. This assay cross-reacts 0.24% with Human Leptin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse Leptin Immunoassay is a 4.5 hour solid phase ELISA designed to measure soluble mouse leptin in cell culture supernates, serum*, and plasma. It contains recombinant mouse leptin expressed in E. coli and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse leptin accurately. Results obtained measuring natural mouse leptin showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse leptin.

*Pregnant mouse serum from the second trimester of pregnancy through post-partum day 1 require acid/urea activation of mouse leptin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	182	693	1803	187	654	1736
Standard Deviation	7.9	22.6	67.9	14.2	30.5	87.4
CV%	4.3	3.3	3.8	7.6	4.7	5

Recovery

The recovery of mouse/rat Leptin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Mouse Cell Culture Supernates (n=5)	101	85-120
Mouse EDTA Plasma (n=4)	92	78-105
Mouse Heparin Plasma (n=4)	95	81-105
Mouse Serum (n=18)	108	81-122
Rat Cell Culture Supernates (n=4)	114	103-125
Rat EDTA Plasma (n=7)	96	83-114
Rat Serum (n=10)	92	81-115

Linearity

To assess the linearity of the assay, four or more samples containing and/or spiked with high concentrations of mouse/rat Leptin in each matrix were diluted with Calibrator Diluent and then assayed.

Mouse/Rat Leptin/OB ELISA Linearity

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