Human VEGF R1/Flt-1 Quantikine ELISA Kit

Human VEGF R1/Flt-1 Quantikine ELISA Kit Summary

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货号：DVR100C

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Cell Lysates (10 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (100 uL)

Sensitivity

8.46 pg/mL

Assay Range

31.3 - 2,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva)

Specificity

Natural and recombinant human VEGF R1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human VEGF R1/Flt-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VEGF R1 in cell culture supernates, cell lysates, serum, plasma, and saliva. It contains NS0-expressed recombinant human VEGF R1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VEGF R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF R1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	207	610	1207	207	616	1205
Standard Deviation	5.16	14.9	35.6	13.9	38.9	74
CV%	2.5	2.4	2.9	6.7	6.3	6.1

Recovery

The recovery of human VEGF R1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	99	87-110
Cell Lysis Buffer (n=4)	94	86-108
EDTA Plasma (n=4)	92	84-98
Heparin Plasma (n=4)	89	81-96
Saliva (n=4)	109	96-124
Serum (n=4)	104	93-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human VEGF R1 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

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