Mouse Erythropoietin Quantikine ELISA Ki

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Mouse Erythropoietin Quantikine ELISA Kit

Mouse Erythropoietin Quantikine ELISA Kit Summary

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货号：MEP00B

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), Heparin Plasma (25 uL)

Sensitivity

46.9 pg/mL

Assay Range

47.0 - 3,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, Heparin Plasma)

Specificity

Natural and recombinant mouse Epo. Recombinant rat Epo has 100% cross-reactivity in this kit. This kit is not validated for use with rat samples, because rat sample dilution is not linear.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse Epo Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Epo in cell culture supernates, tissue homogenates, serum, and plasma. It contains antibodies raised against recombinant Epo and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Epo showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that the Quantikine Mouse Epo kit can be used to determine relative mass values for naturally occurring mouse Epo.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	196	258	704	180	239	700
Standard Deviation	7.7	7.8	31.2	17.5	15.3	17.5
CV%	3.9	3	4.4	9.7	6.4	2.5

Recovery

The recovery of Epo spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=7)	110	102-117
Heparin Plasma (n=8)	103	90-120
Serum (n=9)	93	85-114
Tissue Homogenates (n=3)	94	80-111

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of Epo in each matrix were diluted with Calibrator Diluent and then assayed.

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