Human CCL3/MIP-1 alpha Quantikine ELISA

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Human CCL3/MIP-1 alpha Quantikine ELISA Kit Summary

Human CCL3/MIP-1 alpha Quantikine ELISA Kit Summary

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货号：DMA00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)

Sensitivity

10 pg/mL

Assay Range

31.2 - 1,000 pg/mL (Cell Culture Supernates), 46.9 - 1,500 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human MIP-1 alpha

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MIP-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MIP-1 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-1 alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human MIP-1 alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	116	442	726	53.6	313	623
Standard Deviation	3.3	5.9	14	3.7	13.4	25.7
CV%	2.8	1.3	1.9	6.9	4.3	4.1

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	140	688	1084	50.7	306	613
Standard Deviation	12.5	60.4	55.4	5.9	15.1	37.8
CV%	8.9	8.8	5.1	11.6	4.9	6.2

Recovery

The recovery of MIP-1 alpha spiked to different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	100	88-117
Citrate Plasma (n=5)	94	85-104
EDTA Plasma (n=5)	96	81-109
Heparin Plasma (n=5)	98	82-121
Serum (n=5)	95	87-113

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