Human TGF-beta 2 Quantikine ELISA Kit

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Human TGF-beta 2 Quantikine ELISA Kit

Human TGF-beta 2 Quantikine ELISA Kit Summary

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货号：DB250

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (125 uL), Serum (125 uL), EDTA Plasma (125 uL), Heparin Plasma (125 uL), Citrate Plasma (125 uL)

Sensitivity

7 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human TGF-beta 2 and natural porcine TGF-beta 2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human TGF-beta 2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure activated TGF-beta 2 in cell culture supernates, serum, and plasma. The Quantikine Human TGF-beta 2 Immunoassay contains CHO cell-derived recombinant human TGF-beta 2 and antibodies raised against the recombinant protein. The assay has been shown to quantitate the recombinant factor accurately. Results obtained using human TGF-beta 2 showed linear curves that were parallel to the standard curves obtained using the recombinant TGF-beta 2 kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TGF-beta 2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	194	509	1044	196	500	1015
Standard Deviation	13.8	27	28.3	9.8	21.5	43.6
CV%	7.1	5.3	2.7	5	4.3	4.3

Recovery

To assess recovery of the assay, samples of various matrices were spiked with three concentrations of TGF-beta 2, activated, and measured in the assay.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	101	89-113
Citrate Plasma (n=5)	107	93-116
EDTA Plasma (n=5)	101	95-111
Heparin Plasma (n=5)	102	89-113
Serum (n=5)	86	73-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of TGF-beta 2, activated and then serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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