Human sTfR Quantikine IVD ELISA Kit

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Human sTfR Quantikine IVD ELISA Kit

Human sTfR Quantikine IVD ELISA Kit Summary

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货号：DTFR1

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

2.5 hours

Sample Type & Volume Required Per Well

Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Citrate Plasma (20 uL)

Sensitivity

0.5 nmol/L

Assay Range

3.0 - 80 nmol/L (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Measures human sTfR levels in various samples

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

Interference observed with other substances.

Product Summary

The Quantikine IVD Human sTfR Immunoassay is designed for the quantitative determination of soluble transferrin receptor (sTfR) concentrations in human serum and plasma as an aid in the diagnosis of Iron-Deficiency Anemia (IDA), especially for the differential diagnosis of IDA and Anemia of Chronic Disease (ACD). For In Vitro Diagnostic Use.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	60	60	60
Mean	6.9	18.2	40.2	10.9	25.7	67.2
Standard Deviation	0.3	1.3	2.5	0.7	1.4	3.8
CV%	4.3	7.1	6.2	6.4	5.4	5.7

Recovery

The recovery of sTfR spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Citrate Plasma (n=5)	112	105-121
EDTA Plasma (n=5)	103	97-112
Heparin Plasma (n=5)	106	101-112
Serum (n=5)	97	95-100

Linearity

To assess the linearity of the assay, five samples containing high concentrations of sTfR were diluted with Specimen Diluent and evaluated.

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