Human Pro-MMP-1 Quantikine ELISA Kit

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Human Pro-MMP-1 Quantikine ELISA Kit

Human Pro-MMP-1 Quantikine ELISA Kit Summary

货号：DMP100 规格：96T

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (100 uL), Heparin Plasma (100 uL)

Sensitivity

0.095 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)

Specificity

Natural and recombinant human Pro-MMP-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Pro-MMP-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Pro-MMP-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Pro-MMP-1 and antibodies raised against the recombinant factor. The antibodies will not recognize recombinant human active MMP-1 or TIMP bound MMP-1. Natural human Pro-MMP-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative mass values of natural human Pro-MMP-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	0.494	1.65	3.39	0.561	1.73	3.5
Standard Deviation	0.022	0.065	0.177	0.065	0.153	0.27
CV%	4.5	4	5.2	11.7	8.8	7.7

Serum, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	0.702	2.13	4.43	0.738	2.34	4.66
Standard Deviation	0.039	0.13	0.241	0.077	0.176	0.312
CV%	5.6	6.1	5.4	10.4	7.5	6.7

Recovery

The recovery of Pro-MMP-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	103	98-112
Heparin Plasma (n=5)	92	85-102
Serum (n=5)	97	87-112

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Pro-MMP-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line

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