Human S100A8/S100A9 Heterodimer Quantiki

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Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit

Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit Summary

Catalog #DS8900

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (50 uL), Human Milk (10 uL), Fecal Extract (50 uL)

Sensitivity

0.215 ng/mL

Assay Range

0.6 - 40 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

Specificity

Natural and recombinant human S100A8/S100A9 Heterodimer.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human S100A8/S100A9 Heterodimer Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human S100A8/S100A9 Heterodimer in cell culture supernates, tissue lysastes, serum, plasma, saliva, urine, human milk, and fecal extract. It contains E. coli-expressed recombinant human S100A8/S100A9 Heterodimer and antibodies raised against the recombinant factor. Results obtained using natural human S100A8/S100A9 Heterodimer showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human S100A8/S100A9 Heterodimer.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	5.64	12.1	25.3	6.09	12.6	24.6
Standard Deviation	0.155	0.37	1.14	0.352	0.606	0.792
CV%	2.7	3.1	4.5	5.8	4.8	3.2

Recovery

The recovery of human S100A8/S100A9 Heterodimer spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	85	79-96
Cell Lysis Buffer (n=3)	94	80-104
Human Milk (n=4)	96	83-103
Urine (n=4)	100	91-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human S100A8/S100A9 Heterodimer were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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