Porcine IL-1 beta/IL-1F2 Quantikine ELIS

返回产品中心

Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary

货号：PLB00B

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 uL), Serum (50 uL), Heparin Plasma (50 uL)

Sensitivity

13.6 pg/mL

Assay Range

39.1 - 2,500 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)

Specificity

Natural and recombinant porcine IL-1 beta

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Porcine IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mature porcine IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant porcine IL-1 beta. Results obtained using natural porcine IL-1 beta show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-1 beta.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	136	348	780	141	338	783
Standard Deviation	9.8	17	40.9	12.2	20.5	31.7
CV%	7.2	4.9	5.2	8.7	6.1	4

Recovery

The recovery of porcine IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	106	102-109
Heparin Plasma (n=4)	111	98-117
Serum (n=4)	111	102-117

Linearity

To assess the linearity of the assay, samples containing and/or spiked with various concentrations of porcine IL-1 beta in each matrix were diluted with Calibrator Diluent and then assayed.

Mouse Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit