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Mouse/Rat IL-33 Quantikine ELISA Kit

    

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Mouse/Rat IL-33 Quantikine ELISA Kit Summary

货号:M3300

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
14.3 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse and rat IL-33
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat IL-33 immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-33 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature IL-33 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate both recombinant mouse and rat IL-33. Results obtained using natural IL-33 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-33.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 41 41 41
Mean 58.2 298 630 57.6 282 599
Standard Deviation 5.6 17.4 33.5 5.89 16.3 30
CV% 9.6 5.8 5.3 10.2 5.8 5

Recovery

The recovery of IL-33 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=4) 104 91-116
Mouse EDTA Plasma (n=4) 102 96-113
Mouse Heparin Plasma (n=4) 99 83-111
Mouse Serum (n=4) 90 81-103
Rat Cell Culture Supernates (n=4) 104 94-116
Rat EDTA Plasma (n=4) 93 83-100
Rat Heparin Plasma (n=4) 93 87-99
Rat Serum (n=4) 93 86-101

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