Rat IFN-gamma Quantikine ELISA Kit

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Rat IFN-gamma Quantikine ELISA Kit

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Rat IFN-gamma Quantikine ELISA Kit Summary

Catalog Size / Price
RIF00 1 Kit / Please Inquire
SRIF00 1 Pack (6 Plates) / Please Inquire

PRIF00 1 Pack (50 Plates) / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Specificity

Natural and recombinant rat IFN-gamma

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Rat IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IFN-gamma levels in cell culture supernates and serum. It contains E. coli-expressed recombinant rat IFN-gamma and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant rat IFN-gamma accurately. Results obtained using natural rat IFN-gamma showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IFN-gamma.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	86.9	215	1159	82.8	205	1135
Standard Deviation	3.5	4.4	23	8	14.5	48
CV%	4	2	2	9.7	7.1	4.2

Recovery

The recovery of rat IFN-gamma spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	108	89-119
Serum (n=6)	100	89-111

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of rat IFN-gamma in each matrix were diluted with Calibrator Diluent and then assayed.

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