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Mouse GM-CSF Quantikine ELISA Kit

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Mouse GM-CSF Quantikine ELISA Kit Summary

Catalog        Size / Price
MGM00       1 Kit (2 Plates) / Please Inquire
SMGM00     1 Pack (6 Plates) / Please Inquire

PMGM00     1 Pack (50 Plates) / Please Inquire

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Sensitivity
5.8 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse GM-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse GM-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse GM-CSF in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse GM-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse GM-CSF accurately. Results obtained using natural mouse GM-CSF showed dose-response curves that were parallel to the standard curves obtained using the recombinant standards. These results indicate that this kit can be used to determine relative mass values for natural mouse GM-CSF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 18.5 67.2 219 20 71.7 243
Standard Deviation 0.7 2.1 6.9 1 3.8 11.2
CV% 3.8 3.1 3.2 5 5.3 4.6

Recovery

The recovery of mouse GM-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=8) 96 82-114
Serum (n=7) 94 82-112

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