Human Growth Hormone Quantikine ELISA Ki

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Human Growth Hormone Quantikine ELISA Kit

Human Growth Hormone Quantikine ELISA Kit Summary

Catalog # Availability Size / Price
DGH00 1 Kit / Please Inquire
SGH00 1 Pack (6 Plates) / Please Inquire

PDGH00 1 Pack (50 Plates) / Please Inquire

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

7.18 pg/mL

Assay Range

25.0 - 1,600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Growth Hormone

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human Growth Hormone Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Growth Hormone in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Growth Hormone and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Growth Hormone showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Growth Hormone.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	201	595	1091	209	623	1152
Standard Deviation	9.4	18.8	26	18.2	45.6	63.6
CV%	4.7	3.2	2.4	8.7	7.3	5.5

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	197	586	1092	215	639	1189
Standard Deviation	8	13.8	29.8	20.2	49.5	82.2
CV%	4.1	2.4	2.7	9.4	7.7	6.9

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