Human Fetuin A Quantikine ELISA Kit

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Human Fetuin A Quantikine ELISA Kit

Human Fetuin A Quantikine ELISA Kit Summary

货号：DFTA00 规格：96T

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

1.74 ng/mL

Assay Range

7.8 - 500 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Fetuin A

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Fetuin A Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Fetuin A in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Fetuin A and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Fetuin A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Fetuin A.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	45	147	344	47.1	149	334
Standard Deviation	3.32	10.6	19.6	3	10.7	28.2
CV%	7.4	7.2	5.7	6.4	7.2	8.5

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	43.3	124	264	44.9	127	269
Standard Deviation	1.69	6.08	10.6	3.67	9.34	22.6
CV%	3.9	4.9	4	8.2	7.3	8.4

Recovery

The recovery of Fetuin A spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	103	93-109

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Fetuin A were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Product Summary

Precision

Recovery

Linearity

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