Mouse/Rat TNF RII/TNFRSF1B Quantikine EL

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Mouse/Rat TNF RII/TNFRSF1B Quantikine ELISA Kit

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Mouse/Rat TNF RII/TNFRSF1B Quantikine ELISA Kit Summary

货号：MRT20

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL)

Sensitivity

5 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)

Specificity

Natural and recombinant mouse and rat TNF RII

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat TNF RII Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat TNF RII in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse TNF RII and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant protein accurately. Results obtained using natural mouse or rat TNF RII showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse or rat TNF RII.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	37.2	112	472	38.6	119	500
Standard Deviation	1.5	4.4	21	1.4	3.5	14.7
CV%	4	3.9	4.4	3.6	2.9	2.9

Recovery

The recovery of TNF RII spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Mouse Cell Culture Supernates (n=5)	102	83-119
Mouse EDTA Plasma (n=5)	95	80-119
Mouse Serum (n=5)	99	91-105
Rat Cell Culture Supernates (n=5)	98	85-116
Rat EDTA Plasma (n=5)	95	83-112
Rat Serum (n=5)	97	84-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TNF RII were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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