The Quantikine Human Amyloid beta (aa1-42) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-42) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-42) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta 
(aa1-42) showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-42).

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

The recovery of human Amyloid beta (aa1-42) spiked to levels throughout the range of the assay in various matrices was evaluated.

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta (aa1-42) were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.