Rat IL-2 Quantikine ELISA Kit

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Rat IL-2 Quantikine ELISA Kit

Rat IL-2 Quantikine ELISA Kit Summary

Catalog Size / Price
R2000 1 Kit (2 Plates) / Please Inquire
SR2000 1 Pack (6 Plates) / Please Inquire

PR2000 1 Pack (50 Plates) / Please Inquire

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Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Specificity

Natural and recombinant rat IL-2

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Rat IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-2 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural rat IL-2 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	130	478	1491	139	495	1550
Standard Deviation	4.2	10.5	31.9	13.8	30.8	85.4
CV%	3.2	2.2	2.1	9.9	6.2	5.5

Recovery

The recovery of rat IL-2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=8)	110	93-120
EDTA Plasma (n=4)	102	90-117
Heparin Plasma (n=4)	100	92-116
Serum (n=14)	96	83-114

Linearity

To assess the linearity of the assay, four or more samples containing and/or spiked with various concentrations of rat IL-2 were diluted with Calibrator Diluent and then assayed.

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