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Human Haptoglobin Quantikine ELISA Kit
Human Haptoglobin Quantikine ELISA Kit Summary
货号：DHAPG0
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (20 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (20 uL)
Sensitivity
0.529 ng/mL
Assay Range
3.1 - 200 ng/mL (Serum-free Cell Culture Supern, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural human haptoglobin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays
to determine inter-assay precision. Assays were performed by at least
three technicians using two lots of kit components
Serum-free Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 18.7 | 58.3 | 116 | 18.8 | 57.8 | 113 |
| Standard Deviation | 0.69 | 1.42 | 3.66 | 1.4 | 3.47 | 6.3 |
| CV% | 3.7 | 2.4 | 3.2 | 7.4 | 6 | 5.6 |
Recovery
The recovery of human haptoglobin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Serum-free Cell Culture Media (n=4) | 101 | 94-107 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human haptoglobin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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