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Mouse Periostin/OSF-2 Quantikine ELISA Kit

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Mouse Periostin/OSF-2 Quantikine ELISA Kit

Catalog #	Availability	Size / Price
MOSF20	Contact your distributor for availability.	1 Kit / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.065 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse Periostin

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse Periostin/OSF-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Periostin in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse Periostin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Periostin. Results obtained using natural mouse Periostin showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Periostin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	0.6	0.833	4.69	0.629	0.867	4.84
Standard Deviation	0.021	0.035	0.117	0.056	0.067	0.26
CV%	3.5	4.2	2.5	8.9	7.7	5.4

Recovery

The recovery of mouse Periostin spiked to three levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=6)	95	81-117

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse Periostin in each matrix were diluted with Calibrator Diluent and assayed.

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