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Human TNF-alpha Quantikine ELISA Kit
Human TNF-alpha Quantikine ELISA Kit Summary
Catalog Size
DTA00D 1 Kit / Please Inquire
STA00D 1 Pack (6 Plates) / Please Inquire
PDTA00D 1 Pack (50 Plates) / Please Inquire
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
6.23 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human TNF-alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure human TNF-alpha in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human TNF-alpha and antibodies raised against this protein. It has been shown to accurately quantitate the recombinant factor. Results obtained with naturally occurring TNF-alpha samples showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TNF-alpha.
Recovery
The recovery of human TNF-alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 98-110 |
| EDTA Plasma (n=4) | 90 | 75-101 |
| Heparin Plasma (n=4) | 90 | 82-95 |
| Serum (n=4) | 88 | 79-98 |
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