Mouse/Rat/Porcine/Canine TGF-beta 1 Quan

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Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit

Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit Summary

Catalog Size / Price
MB100B 1 Kit / Please Inquire
SMB100B 1 Pack (6 Plates) / Please Inquire

PMB100B 1 Pack (50 Plates) / Please Inquire

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (40 uL), EDTA Plasma (40 uL), Heparin Plasma (40 uL)

Sensitivity

15.4 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant TGF-beta 1. This kit also recognizes human TGF-beta 1.2.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse/Rat/Porcine/Canine TGF-beta 1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TGF-beta 1 in acid activated cell culture supernates, serum, and plasma. It contains recombinant TGF-beta 1 expressed by CHO cells and has been shown to quantitate the recombinant factor accurately. Results obtained using natural TGF-beta 1 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit will provide accurate quantitation for both recombinant and natural TGF-beta 1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	28	37	30
Mean	78	180	695	79	185	730
Standard Deviation	2.3	3.4	17.3	7.2	17.2	46.8
CV%	2.9	1.9	2.5	9.1	9.3	6.4

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	22	21	22
Mean	88	198	816	97	209	826
Standard Deviation	3	4.9	19.6	8.1	17.3	47
CV%	3.4	2.5	2.4	8.4	8.3	5.7

Recovery

The recovery of TGF-beta 1 spiked to levels throughout the range of the assay followed by activation was evaluated.

Sample Type	Average % Recovery	Range %
Mouse Cell Culture Supernates (n=4)	105	83-114
Porcine Cell Culture Supernates (n=2)	109	102-118
Rat Cell Culture Supernates (n=3)	112	93-125

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