Human Total Adiponectin/Acrp30 Quantikin

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Human Total Adiponectin/Acrp30 Quantikine ELISA Kit

Human Total Adiponectin/Acrp30 Quantikine ELISA Kit Summary

Catalog Size / Price
DRP300 1 Kit / Please Inquire
SRP300 1 Pack (6 Plates) / Please Inquire

PDRP300 1 Pack (50 Plates) / Please Inquire

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.891 ng/mL

Assay Range

3.9 - 250 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human total Adiponectin (low, middle, and high molecular weight)

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Total Adiponectin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total (low, middle, and high molecular weight) human Adiponectin in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Adiponectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Adiponectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Adiponectin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	19.8	69.9	143	20.5	74.4	157
Standard Deviation	0.5	2.4	6.76	1.4	4.3	10.8
CV%	2.5	3.4	4.7	6.8	5.8	6.9

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	12.5	45.3	91.5	12.7	47.1	100
Standard Deviation	0.41	1.27	4.56	1	2.8	6.7
CV%	3.2	2.8	5	7.9	5.9	6.7

Recovery

The recovery of Adiponectin spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	88-112

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