Human CXCL10/IP-10 Quantikine ELISA Kit

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Human CXCL10/IP-10 Quantikine ELISA Kit

Human CXCL10/IP-10 Quantikine ELISA Kit Summary

货号：DIP100

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 uL), Serum (75 uL), EDTA Plasma (75 uL), Heparin Plasma (75 uL), Saliva (10 uL), Urine (100 uL)

Sensitivity

4.46 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)

Specificity

Natural and recombinant human IP-10

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IP-10 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IP-10 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IP-10 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IP-10 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring IP-10.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	70.3	174	342	80.7	194	362
Standard Deviation	2.07	5.15	10.74	7.91	13.48	24.27
CV%	2.9	3	3.1	9.8	6.9	6.7

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	67.4	168	334	70.7	182	366
Standard Deviation	3.12	4.99	11.15	6.23	11.12	18.93
CV%	4.6	3	3.3	8.8	6.1	5.2

Recovery

The recovery of IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	95	88-101
EDTA Plasma (n=5)	99	90-109
Heparin Plasma (n=5)	99	87-113
Serum (n=5)	99	88-112

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IP-10 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human CXCL10/IP-10/CRG-2 ELISA Linearity

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