Mouse CXCL1/KC Quantikine ELISA Kit

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Mouse CXCL1/KC Quantikine ELISA Kit

Mouse CXCL1/KC Quantikine ELISA Kit Summary

货号：MKC00B

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL)

Sensitivity

2 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum)

Specificity

Natural and recombinant mouse KC

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse KC Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse KC in cell culture supernates and mouse serum. It contains E. coli-expressed recombinant mouse KC and antibodies raised against the recombinant factor. This Immunoassay has been shown to quantitate the recombinant mouse KC accurately. Results obtained using natural mouse KC showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse KC.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	34.9	169	530	35	183	521
Standard Deviation	1.9	5.2	26.3	2.1	18	15.6
CV%	5.4	3.1	5	6	9.8	3

Recovery

The recovery of mouse KC spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=7)	98	89-103
Serum (n=9)	95	83-104

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse KC in each matrix were diluted with Calibrator Diluent and then assayed.

Mouse CXCL1/GRO alpha/KC/CINC-1 ELISA Linearity

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