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Rat TNF-alpha Quantikine ELISA Kit
Rat TNF-alpha Quantikine ELISA Kit Summary
货号：RTA00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Specificity
Natural and recombinant rat TNF-alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 65 | 232 | 593 | 63 | 246 | 656 |
| Standard Deviation | 3.3 | 5.1 | 12.5 | 6.1 | 23.6 | 57.6 |
| CV% | 5.1 | 2.2 | 2.1 | 9.7 | 9.6 | 8.8 |
Recovery
The recovery of rat TNF-alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 101 | 93-119 |
| EDTA Plasma (n=5) | 96 | 88-104 |
| Heparin Plasma (n=4) | 80 | 73-84 |
| Serum (n=8) | 93 | 83-105 |
Linearity
To assess the linearity of the assay, samples spiked with various concentrations of rat TNF-alpha were diluted with Calibrator Diluent and then assayed. Results from typical sample dilutions are shown.
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