Human C-Reactive Protein/CRP Quantikine

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Human C-Reactive Protein/CRP Quantikine ELISA Kit

Human C-Reactive Protein/CRP Quantikine ELISA Kit Summary

货号：DCRP00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

0.022 ng/mL

Assay Range

0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human CRP

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human CRP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CRP in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CRP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CRP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CRP.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	4.79	8.66	18.9	4.84	8.66	17.5
Standard Deviation	0.21	0.33	1.57	0.29	0.61	1.15
CV%	4.4	3.8	8.3	6	7	6.6

Recovery

The recovery of CRP spiked to levels throughout the range of the assay in cell culture media was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	100	92-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CRP were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human C-Reactive Protein/CRP ELISA Linearity

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