Human HGF Quantikine ELISA Kit

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Human HGF Quantikine ELISA Kit

Human HGF Quantikine ELISA Kit Summary

货号：DHG00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.25 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Citrate Plasma (50 uL)

Sensitivity

40 pg/mL

Assay Range

125.0 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)

Specificity

Natural and recombinant human HGF

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human HGF Immunoassay is a 4.25 or 4.5 hour solid phase ELISA that is designed to measure HGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human pro-HGF and antibodies raised against the recombinant factor and has been shown to quantitate recombinant human HGF accurately. Results obtained using natural HGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	339	999	3759	363	967	3790
Standard Deviation	23.7	56.2	154	25.8	81.2	205
CV%	7	5.6	4.1	7.1	8.4	5.4

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	425	991	3869	409	1058	4058
Standard Deviation	30.2	43.2	234	29.4	75.1	264
CV%	7.1	4.4	6	7.2	7.1	6.5

Recovery

The recovery of HGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	92	86-101
Citrate Plasma (n=5)	105	99-114
EDTA Plasma (n=5)	100	85-111
Serum (n=5)	99	91-108

Linearity

To assess the linearity of the assay, five samples of each sample type were spiked with high concentrations of HGF and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human HGF ELISA Linearity

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