Mouse IL-4 Quantikine ELISA Kit

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Mouse IL-4 Quantikine ELISA Kit

Mouse IL-4 Quantikine ELISA Kit Summary

货号：M4000B

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL)

Sensitivity

2 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, Serum)

Specificity

Natural and recombinant mouse IL-4

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse IL-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-4 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-4 accurately. Results obtained using natural mouse IL-4 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-4.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	20.1	58.6	199	20.7	58.8	203
Standard Deviation	1.3	1.8	8.9	1.2	2.7	10.6
CV%	6.5	3.1	4.5	5.8	4.6	5.2

Recovery

The recovery of mouse IL-4 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=8)	95	84-109
Serum (n=7)	95	88-118

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-4 in each matrix were diluted with Calibrator Diluent and then assayed.

Mouse IL-4 ELISA Linearity

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