Human Free BDNF Quantikine ELISA Kit

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Human Free BDNF Quantikine ELISA Kit

Human Free BDNF Quantikine ELISA Kit Summary

货号：DBD00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL), Citrate Plasma (50 uL)

Sensitivity

20 pg/mL

Assay Range

62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human BDNF

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure human BDNF in cell culture supernates, serum, and plasma. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural human BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human BDNF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	476	1258	2175	528	1338	2287
Standard Deviation	24	48.2	135.7	59.7	102.3	186
CV%	5	3.8	6.2	11.3	7.6	8.1

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	334	825	1339	358	905	1517
Standard Deviation	15.6	28.3	56.5	36.8	86.8	121.6
CV%	4.7	3.4	4.2	10.3	9.6	8.1

Recovery

The recovery of BDNF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	96	83-105
Platelet-poor Citrate Plasma (n=5)	99	87-120
Platelet-poor EDTA Plasma (n=5)	96	89-115
Platelet-poor Heparin Plasma (n=5)	93	81-119
Serum (n=5)	100	85-116

Linearity

To assess the linearity of the assay, five samples containing or spiked with high concentrations of BDNF in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Free BDNF ELISA Linearity

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