Mouse IL-13 Culture Media Quantikine ELI

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Mouse IL-13 Culture Media Quantikine ELISA

Mouse IL-13 Culture Media Quantikine ELISA Summary

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货号：M1300CB

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL)

Sensitivity

1.5 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates)

Specificity

Natural and recombinant mouse IL-13

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-13 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-13 levels in cell culture supernates. It contains E. coli-expressed recombinant mouse IL-13 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-13 accurately. Results obtained using natural mouse IL-13 showed dose curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-13.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	33.9	100	432	31.6	93.4	405
Standard Deviation	1.1	2.7	11.2	2.5	7.2	17.7
CV%	3.2	2.7	2.6	7.9	7.7	4.4

Recovery

The recovery of mouse IL-13 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=13)	99	89-119

Linearity

To assess the linearity of the assay, samples containing and/or spiked with mouse IL-13 were diluted with Calibrator Diluent and assayed.

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