Human TIMP-1 Quantikine ELISA Kit

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Human TIMP-1 Quantikine ELISA Kit

Human TIMP-1 Quantikine ELISA Kit Summary

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货号：DTM100

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL)

Sensitivity

0.08 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)

Specificity

Natural and recombinant human TIMP-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human TIMP-1 Immunoassay is a 3.5 hour solid phase ELISA designed to measure TIMP-1 concentrations in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human TIMP-1 and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human TIMP-1 accurately. Results obtained using natural TIMP-1 showed linear curves that were parallel to the standard curve obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural TIMP-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	0.48	1.27	6.95	0.51	1.28	6.9
Standard Deviation	0.02	0.05	0.35	0.02	0.05	0.34
CV%	4.2	3.9	5	3.9	3.9	4.9

Recovery

The recovery of TIMP-1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	102	94-113
EDTA Plasma (n=5)	99	93-108
Heparin Plasma (n=5)	99	89-108
Saliva (n=4)	105	89-121
Serum (n=5)	98	87-102

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of TIMP-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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