Human IL-1ra/IL-1F3 Quantikine ELISA Kit

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Human IL-1ra/IL-1F3 Quantikine ELISA Kit

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Human IL-1ra/IL-1F3 Quantikine ELISA Kit Summary

货号：DRA00B

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)

Sensitivity

18.3 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IL-1ra

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human IL-1ra Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-1ra in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human IL-1ra as well as antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-1ra. Results obtained using natural human IL-1ra showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1ra.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	66.9	607	1627	82.5	646	1663
Standard Deviation	4.88	30.2	60	9.04	51.4	111
CV%	7.3	5	3.7	11	8	6.7

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	64.9	538	1343	56.2	469	1243
Standard Deviation	3.67	22.1	55.7	5.79	31.9	70.4
CV%	5.6	4.1	4.1	10.3	6.8	5.7

Recovery

The recovery of IL-1ra spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	106	96-115
EDTA Plasma (n=4)	86	66-102
Heparin Plasma (n=4)	93	82-106
Serum (n=4)	90	81-99

Linearity

To assess the linearity of the assay, samples containing high concentrations of IL-1ra were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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