Human CXCL13/BLC/BCA-1 Quantikine ELISA

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Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit Summary

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货号：DCX130

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (25 uL)

Sensitivity

3.97 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)

Specificity

Natural and recombinant human BLC/BCA-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human BLC/BCA-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BLC/BCA-1 in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant human BLC/BCA-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BLC/BCA-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring BLC/BCA-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	50.2	136	294	54	138	285
Standard Deviation	2.2	4.5	8	5.1	13.2	24.8
CV%	4.3	3.3	2.7	9.4	9.6	8.7

Recovery

The recovery of natural BLC/BCA-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	87-110
EDTA Plasma (n=4)	99	92-107
Heparin Plasma (n=4)	101	92-115
Serum (n=4)	99	86-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of natural BLC/BCA-1 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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