Mouse IL-12 p70 Quantikine ELISA Kit

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Mouse IL-12 p70 Quantikine ELISA Kit

Mouse IL-12 p70 Quantikine ELISA Kit Summary

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货号：M1270

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL)

Sensitivity

2.5 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, Serum)

Specificity

Natural and recombinant mouse IL-12 p70

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-12 p70 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-12 p70 in cell culture supernates and mouse serum. It contains recombinant mouse IL-12 expressed in Sf 21 insect cells using a baculovirus expression vector and antibodies raised against recombinant mouse IL-12. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-12 showed linear curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12 p70.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	36	44	36
Mean	28	68	193	20	56	230
Standard Deviation	2.5	6.5	19.4	2.3	6.2	20
CV%	8.9	9.6	10.1	11.5	11.1	8.7

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

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