Mouse/Rat FGF-21 Quantikine ELISA Kit

返回产品中心

Mouse/Rat FGF-21 Quantikine ELISA Kit

Mouse/Rat FGF-21 Quantikine ELISA Kit Summary

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

货号：MF2100

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

13.4 pg/mL

Assay Range

31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse and rat FGF-21

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat FGF-21 immunoassay is a 4.5 hour solid-phase ELISA designed to measure FGF-21 in mouse or rat cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse FGF-21 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse FGF-21. Results obtained using natural mouse or rat FGF-21 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural FGF-21.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	118	317	1032	120	302	977
Standard Deviation	7.35	13.5	25.2	8.91	18	65.9
CV%	6.2	4.3	2.4	7.4	6	6.7

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	84.4	226	754	94.5	231	745
Standard Deviation	5.24	9.6	19.7	6.61	10.8	31
CV%	6.2	4.2	2.6	7	4.7	4.2

Recovery

The recovery of FGF-21 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Mouse Cell Culture Samples (n=6)	96	88-101
Mouse EDTA Plasma (n=4)	97	86-104
Mouse Heparin Plasma (n=4)	93	83-101
Mouse Serum (n=4)	95	83-103
Rat Cell Culture Samples (n=2)	91	87-95
Rat EDTA Plasma (n=4)	101	93-119
Rat Heparin Plasma (n=4)	97	94-104
Rat Serum (n=4)	100	93-108

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of FGF-21 in each matrix were diluted with the appropriate Calibrator Diluent and assayed.

Human PlGF Quantikine ELISA Kit

Human sTNF RII/TNFRSF1B Quantikine ELISA Kit

返回 产品中心