Human PlGF Quantikine ELISA Kit

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Human PlGF Quantikine ELISA Kit

Human PlGF Quantikine ELISA Kit Summary

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货号：DPG00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)

Sensitivity

7 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)

Specificity

Natural and recombinant human PlGF

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human PlGF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure human PlGF in cell culture supernates, serum, plasma, and urine. The immunoassay kit contains E. coli-expressed recombinant human PlGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human PlGF accurately. Results obtained using natural human PlGF showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PlGF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	40.9	138	541	46.9	149	555
Standard Deviation	2.4	7.3	38.2	6.4	13.5	32.9
CV%	5.9	5.3	7.1	13.6	9.1	5.9

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	54.3	175	658	55	184	724
Standard Deviation	3.8	6.3	36.9	6.5	20.3	78.9
CV%	7	3.6	5.6	11.8	11	10.9

Recovery

The recovery of PlGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	97	82-107
Citrate Plasma (n=5)	99	93-120
EDTA Plasma (n=5)	102	92-117
Heparin Plasma (n=5)	94	81-113
Serum (n=5)	96	88-118
Urine (n=4)	97	85-112

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of PlGF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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