Endothelin-1 Quantikine ELISA Kit

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Endothelin-1 Quantikine ELISA Kit

Endothelin-1 Quantikine ELISA Kit Summary

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货号：DET100

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (75 uL), Serum (75 uL), EDTA Plasma (75 uL), Heparin Plasma (75 uL), Urine (75 uL)

Sensitivity

0.207 pg/mL

Assay Range

0.4 - 25 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Synthetic and natural Endothelin-1

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Endothelin-1 immunoassay is a 4.5 hour solid phase ELISA designed to measure ET-1 in cell culture supernates, serum, plasma, and urine. It contains synthetic ET-1 and antibodies raised against synthetic ET-1. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring ET-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	3	7.34	14.7	3.05	7.43	14.4
Standard Deviation	0.12	0.17	0.28	0.231	0.438	0.759
CV%	4	2.3	1.9	7.6	5.9	5.3

Recovery

The recovery of Endothelin-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	99	88-108
Human EDTA Plasma (n=4)	93	87-102
Human Heparin Plasma (n=4)	93	85-106
Human Serum (n=4)	98	86-107
Mouse EDTA Plasma (n=4)	99	102-107
Mouse Serum (n=4)	94	86-104
Rat EDTA Plasma (n=4)	99	86-113
Rat Serum (n=4)	98	88-113
Urine (n=4)	91	85-109

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of Endothelin-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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