Human IL-12 p70 Quantikine ELISA Kit

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Human IL-12 p70 Quantikine ELISA Kit

Human IL-12 p70 Quantikine ELISA Kit Summary

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货号：D1200

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL), Urine (200 uL)

Sensitivity

5 pg/mL

Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)

Specificity

Natural and recombinant human IL-12p70

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-12 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-12 in cell culture supernates, serum, plasma, and urine. It contains Sf 21-expressed recombinant human IL-12 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-12 accurately. Results obtained using natural human IL-12 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-12.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	25.7	202	436	19.9	115	223
Standard Deviation	1.6	4.8	10.1	1.9	8.3	13.2
CV%	6.2	2.4	2.3	9.5	7.2	5.9

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	21.2	228	447	21	126	246
Standard Deviation	0.3	3.5	4.9	1.5	5.6	8.1
CV%	1.4	1.5	1.1	7.1	4.4	3.3

Recovery

The recovery of human IL-12 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	90	81-96
Citrate Plasma (n=5)	90	82-94
EDTA Plasma (n=5)	101	96-109
Heparin Plasma (n=5)	99	89-109
Serum (n=5)	100	89-106
Urine (n=5)	89	85-92

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of IL-12 in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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