Human IL-1 alpha/IL-1F1 Quantikine ELISA

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Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary

Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary

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货号：DLA50

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)

Sensitivity

1 pg/mL

Assay Range

3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human IL-1 alpha

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human IL-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 alpha in cell culture supernates, serum, and plasma. It contains recombinant human IL-1 alpha and antibodies raised against recombinant human IL-1 alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-1 alpha showed linear curves that were parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1 alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	28.6	125	185	27.9	123	187
Standard Deviation	0.4	1.4	4	1.2	4.7	6.3
CV%	1.4	1.1	2.2	4.3	3.8	3.4

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	26.9	122	191	20.5	60	121
Standard Deviation	0.4	4.6	6.6	1.7	3.1	5.2
CV%	1.5	3.8	3.5	8.3	5.2	4.3

Recovery

The recovery of IL-1 alpha spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	96	91-104
Citrate Plasma (n=6)	97	71-119
EDTA Plasma (n=6)	104	84-121
Heparin Plasma (n=6)	104	72-126
Serum (n=6)	107	74-127

Linearity

To assess the linearity of the assay, the following biological samples spiked with high concentrations of IL-1 alpha were diluted with the appropriate Calibrator Diluent and then assayed.

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