Human BAFF/BLyS/TNFSF13B Quantikine ELIS

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Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit Summary

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货号：DBLYS0B

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

6.44 pg/mL

Assay Range

62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human BAFF/BLyS

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human BAFF/BLyS Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BAFF/BLyS in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human BAFF/BLyS and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BAFF/BLyS showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BAFF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	433	1000	2343	474	1118	2111
Standard Deviation	28.2	72	78.6	46.8	112	244
CV%	6.5	7.2	3.4	9.9	10	11.6

Recovery

The recovery of BAFF/BLyS spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	96	90-103
EDTA Plasma (n=4)	94	84-101
Heparin Plasma (n=4)	94	89-107
Serum (n=4)	93	84-106

Linearity

Human IL-12 p70 Quantikine ELISA Kit

Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary

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