Mouse Lipocalin-2/NGAL Quantikine ELISA

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Mouse Lipocalin-2/NGAL Quantikine ELISA Kit Summary

Mouse Lipocalin-2/NGAL Quantikine ELISA Kit Summary

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货号：MLCN20

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL), Urine (10 uL)

Sensitivity

8.8 pg/mL

Assay Range

78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)

Specificity

Natural and recombinant mouse Lipocalin-2

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse Lipocalin-2/NGAL Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Lipocalin-2 in cell culture supernates, mouse serum, platelet-poor plasma, and urine. It contains NS0-expressed recombinant mouse Lipocalin-2 and antibodies raised against the recombinant factor. Natural mouse Lipocalin-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural mouse Lipocalin-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	196	476	1534	211	477	1564
Standard Deviation	13.2	19.6	65.9	15.2	28.6	98.5
CV%	6.7	4.1	4.3	7.2	6	6.3

Recovery

The recovery of mouse Lipocalin-2 spiked to three levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=4)	93	82-101

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse Lipocalin-2 were diluted with Calibrator Diluent and then assayed.

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