Human IL-17 Quantikine ELISA Kit Summary

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Human IL-17 Quantikine ELISA Kit Summary

Human IL-17 Quantikine ELISA Kit Summary

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货号：D1700

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)

Sensitivity

15 pg/mL

Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human IL-17

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-17 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-17 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-17 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-17 accurately. Results obtained using natural human IL-17 showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-17.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	131	736	1480	125	408	794
Standard Deviation	5.8	34.6	61.2	9.8	34.3	55.6
CV%	4.4	4.7	4.1	7.8	8.4	7

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	107	604	1217	76.6	272	549
Standard Deviation	7.8	25	63.3	6.5	23.4	43.9
CV%	7.3	4.1	5.2	8.5	8.6	8

Recovery

The recovery of human IL-17 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	97	88-112
Citrate Plasma (n=5)	105	92-116
EDTA Plasma (n=5)	101	90-110
Heparin Plasma (n=5)	101	93-109
Serum (n=5)	98	90-105

Linearity

To assess the linearity of the assay, five samples spiked with high concentrations of IL-17 were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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