Human Proprotein Convertase 9/PCSK9 Quan

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Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit

Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit Summary

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货号：DPC900

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.219 ng/mL

Assay Range

0.6 - 40 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human PCSK9. This assay recognizes free and LDLR-bound PCSK9.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human PCSK9 immunoassay is a 4.5 hour solid phase ELISA designed to measure PCSK9 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human PCSK9 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural PCSK9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring PCSK9.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	4.82	14	27.7	4.64	14.5	27.9
Standard Deviation	0.196	0.795	1.81	0.276	0.629	1.13
CV%	4.1	5.7	6.5	6	4.3	4.1

Recovery

The recovery of PCSK9 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	107	100-111

Linearity

To assess the linearity of the assay, samples containing high concentrations of PCSK9 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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