Human G-CSF Quantikine ELISA Kit Summary

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Human G-CSF Quantikine ELISA Kit Summary

Human G-CSF Quantikine ELISA Kit Summary

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货号：DCS50

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

3.5 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)

Sensitivity

20 pg/mL

Assay Range

39.0 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human G-CSF

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human G-CSF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure G-CSF in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human G-CSF and antibodies raised against the protein. It has been shown to accurately quantitate recombinant human G-CSF. Results obtained using natural human G-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human G-CSF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	177	515	1047	143	818	1514
Standard Deviation	4.5	9.4	22	17	72	77.1
CV%	2.5	1.8	2.1	11.9	8.8	5.1

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	280	827	1696	176	1094	2169
Standard Deviation	7.8	14.1	19.5	7.3	34.6	81.6
CV%	2.8	1.7	1.1	4.1	3.2	3.8

Recovery

The recovery of G-CSF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	94	85-110
Citrate Plasma (n=5)	90	78-111
EDTA Plasma (n=5)	93	80-117
Heparin Plasma (n=5)	87	76-103
Serum (n=5)	88	75-106

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of G-CSF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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